BRF GeMS Research Scientist

BRF GeMS Research Scientist page is loaded
BRF GeMS Research Scientist Apply
locations
London
time type
Full time
posted on
Posted Yesterday
job requisition id
R1695
Salary for this Role:From
£35,000
with benefits, subject to skills and experienceJob Title:BRF GeMS Research ScientistReports to:Graham DuddyClosing Date:12/Jun/2024 23.59 GMTJob Description:BRF GEMS Research ScientistReports to: GeMs Senior Manager Molecular & Cell.This is a full-time, permanent position on Crick terms and conditionsSummaryThe Biological Research Facility (BRF) supports all animal research at the Crick through our comprehensive animal care and use programme. The BRF provides the Crick's laboratory animal resource and technical services. High quality multidisciplinary in-vivo and ex-vivo research support are at the heart of the BRF's philosophy and operations. The BRF is committed to improving animal welfare, scientific quality, care of staff and transparency for stakeholders. The BRF teams integrate husbandry of multiple species within a range of facilities, using state of the art technologies, via a talented and skilled teamAnimal models provide an important means to determine gene function and model disease. The BRF are providing expertise and support for housing and experiment, all underpinned with world-class standards of welfare. Within the BRF, Genetic Modification Services (GeMS) provide a breadth of specialist skills and resources for the design, creation, production, storage and use of genetically altered animals, germplasms and cell lines together with ancillary techniques.The services that GeMS offer are frequently updated, but currently comprise:Molecular biology –the design and production of sequences for targeting strategies, with a focus on CRISPR & novel techniques.Micromanipulation – introduction of gene sequence by electroporation, cytoplasmic or pronuclear microinjection of one cell fertilised embryos, ES cell microinjection of blastocysts or 8 cell embryos and aggregationSurgical expertise– mainly embryo transfer surgery but specialism in other surgical techniques as required as well as training in aseptic techniqueCryopreservation of rodent and aquatic germplasm for archiving and backup of GA linesAssisted Reproductive techniques such as IVF, AI, ICSIRederivation of live and frozen imports & exports to and from the Crick, managing the shipment process, and maintaining the integrity of the health status of the BRF.Murine stem cell culture, maintenance and transfection, derivation and characterisation.The GeMS Research Scientist (RS) post holder will participate in the creation of new animal models, taking a lead role towards the development of a subset of projects. As such, you will be expected develop a detailed understanding of genetic modification and mouse genetics and will be expected to follow breeding progress ensuring that the 3R’s principles for in vivo research (replacement, reduction and refinement) underpins all work that is undertaken.Key responsibilitiesThese include but are not limited to:Assist in the design, generation of reagents and validation of new genetically modified alleles.Perform core molecular biology techniques including LR-PCR, PCR, digital droplet or qPCR, molecular cloning, NGS sequencing technologies, genotyping and sequence analysis.Maintenance of mouse embryonic stem cultures and perform in vitro CRISPR facilitated and classical gene targeting experiments using electroporation, nucleofection and lipofection. Engage in out of hours cell culture work rota.Participate in the creation of new animal models, taking a lead role towards the development of a subset of projects; working towards agreed deadlines, identifying and troubleshooting at each step, seeking support when required.Maintain records, ensuring your own scientific work is always carried out accurately and efficiently, adhering to legal frameworks.Awareness and promotion of all relevant health and safety guidance and legislation.Ensure compliance to appropriate SOPs and best practice.Provide written and verbal reports as required, including those related to animal welfare, production, scientific procedures and health and safety matters.Ordering consumables via a computer ordering system, ensuring value for money at all times and maintaining the budget for that service aspectUse of the Colony Management and other appropriate storage and procedural databases.Using and training of others in all in house centralised database systems appropriately and report any issues with any in house database systems to the Senior Manager and cascading to appropriate working groups.Person SpecificationThe post holder should embody and demonstrate our core Crick values: Bold, Imaginative, Open, Dynamic and Collegiate, in addition to the following:Essential Qualifications, experience and competencies:A degree or equivalent in a biological subject.Experience in a relevant position, particularly in molecular biology and mammalian cell culture.Understanding CRISPR-CAS9 design and associated techniques.Experience of using genome browsers, DNA analysis software and CRISPR CAS9 guide on/off target specificity software.Commitment to continued professional development (CPD).Experience of contributing effectively to the work of a team with a flexible and collaborative approach.Experience or willingness to work with animal models.Able to work independently and drive a project forward through good project planning, time-keeping, organisational and record-keeping skills.An understanding of health and safety requirements in relation to animal care and useGood communication skills, both oral and written with the ability to relay straight forward and complex information to a broad range of stakeholders.Good interpersonal skills enabling the post holder to influence and deliver at all levelsStrong planning skills, the ability to prioritize in the face of competing demands, proven success in delivering work personally (and through others) to tight deadlinesGood decision-making skills with the ability to use initiative and problem solveDesirableDesigning molecular complex cloning strategies. Previous gene targeting/gene editing experience.Design and implementation of genotyping strategies.Previous experience of designing and performing CRISPR-CAS gene editing experiments.Practical experience of NGS technologies.Knowledge and understanding of the requirements of the legislative framework, Animals (Scientific Procedures) Act 1986 (Amended 2012), Human Fertilisation and Embryo Act (#####) and all other relevant legislationFind out what benefits the Crick has to offer:For more information on our great pay and benefits package please click here:
Equality, Diversity & Inclusion:We welcome applications from all backgrounds. We are committed to providing equal employment opportunities, regardless of ethnicity, nationality, gender, sexual orientation, gender identity, religion, pregnancy, age, disability, or civil partnership, marital or family status. We particularly welcome applications from people who are Minority Ethnic as they are currently underrepresented in the Crick at this level.Diversity is essential to excellence in scientific endeavour. It increases breadth and perspective, leading to more innovation and creativity. We want the Crick to be a place where everyone feels valued and where diversity is celebrated and seen as part of the foundation for our Institute’s success.The Crick is committed to creating equality of opportunity and promoting diversity and inclusivity. We all share in the responsibility to actively promote dignity, respect, inclusivity and equal treatment and it is our aim to ensure that these principles are reflected and implemented in all strategies, policies and practices.Read more on our website:
#J-18808-Ljbffr